Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD)
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Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
متن کاملEvaluation of Genetic Variability in a Breeder Flock of Native Chicken Based on Randomly Amplified Polymorphic DNA Markers
A study was undertaken to evaluate the genetic variation in the 10th generation of a breeder flock of native breed selected for high egg and meat production in native fowls breeding station, Mazandaran, Iran. Venous blood samples were collected from 100 birds of both sexes. The RAPD-PCR technique was applied to generate a DNA fingerprint of individuals. Initially, a total of 20 ten-nucleotide a...
متن کاملIDENTIFICATION OF LEISHMANIA SPECIES FROM DIFFERENT PARTS OF IRAN USING A RANDOM AMPLIFIED POLYMORPHIC DNA IN HUMANS, ANIMAL RESERVOIRS AND VECTORS
In this study, we used Random Amplified Polymorphic DNA (RAPD) for identification of 17 isolates of Leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of Iran in the last decade. Fifteen species have been confirmed by isoenzyme characterization by the London School of Hygiene and Tropical Medicine and Shiraz ...
متن کاملRAPD Analysis for Sex Determination in Pistacia vera L.
Random Amplified Polymorphic DNA (RAPD) analysis of Pistacia vera L. was used to distinguishing sex type in progeny plants. Varied genotypes of the pistachio were analyzed by PCR amplification of a ten base primer previously found to be linked to the expression of a 945bp amplification band in female trees and its absence in males. Results revealed that genetic material from cultivar samples am...
متن کاملDifferentiation of Iranian Strains of Brucella spp. by Random Amplification of Polymorphic DNA
101 isolates of Brucella spp. including the reference (n=7) and the Iranian field strains belonging to last years 1961-2003 (n=94) were biotyped and classified into 9 biotypes. Random amplified polymorphic DNA (RAPD) analysis using a ten mer-AP4 primer was generated 72 reproduciable DNA band. The typeability was 100%. The thirteen isolates were classified into five groups, each containing 2-4 b...
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